Biogenesis, architecture and interactions of RNA

Head : Carine Tisné

Team Publications


  1. Barraud P, Tisné C. Cracking the case of m7G modification in human tRNAs (2023). Nat Struct Mol Biol. 30(3):242-243. doi: 10.1038/s41594-023-00937-5.

  2. Charvin M., Halter T., Blanc-Mathieu R., Barraud P. ,Aumont-Nicaise M. ,Parcy F., Navarro L. (2023) Single-cytosine methylation at W-boxes repels binding of WRKY transcription factors through steric hindrance. Plant Physiol. doi: 10.1093/plphys/kiad069

  3. Meynier V, Iannazzo L, Catala M, Oerum S, Braud E, Atdjian C, Barraud P, Fonvielle M, Tisné C, Ethève-Quelquejeu M. Synthesis of RNA-cofactor conjugates and structural exploration of RNA recognition by an m6A RNA methyltransferase (2022). Nucleic Acids Res. ;50(10):5793-5806. doi: 10.1093/nar/gkac354.

  4. Mouhand A, Zargarian L, Belfetmi A, Catala M, Pasi M, Lescop E, Tisné C, Mauffret O. Investigation of the Low-Populated Excited States of the HIV-1 Nucleocapsid Domain (2022) Viruses 14(3):632. doi: 10.3390/v14030632.

  5. Gilmer O, Mailler E, Paillart JC, Mouhand A, Tisné C, Mak J, Smyth RP, Marquet R, Vivet-Boudou V. Structural maturation of the HIV-1 RNA 5' untranslated region by Pr55Gag and its maturation products (2022). RNA Biol; 19(1):191-205. doi: 10.1080/15476286.2021.2021677.

  6. Lyonnais S, Sadiq SK, Lorca-Oró C, Dufau L, Nieto-Marquez S, Escribà T, Gabrielli N, Tan X, Ouizougun-Oubari M, Okoronkwo J, Reboud-Ravaux M, Gatell JM, Marquet R, Paillart JC, Meyerhans A, Tisné C, Gorelick RJ, Mirambeau G. The HIV-1 Nucleocapsid Regulates Its Own Condensation by Phase-Separated Activity-Enhancing Sequestration of the Viral Protease during Maturation (2021) Viruses. 13(11):2312. doi: 10.3390/v13112312.

  7. Gato A, Catala M, Tisné C, Barraud P. A Method to Monitor the Introduction of Posttranscriptional Modifications in tRNAs with NMR Spectroscopy (2021). Methods Mol Biol. 2021;2298:307-323. doi: 10.1007/978-1-0716-1374-0_19.

  8. El Khouri M, Catala M, Seijo B, Chabal J, Dardel F, Tisné C, Ponchon L. Coexpression and Copurification of RNA-Protein Complexes in Escherichia coli. Methods Mol Biol. 2021;2323:67-73. doi: 10.1007/978-1-0716-1499-0_6.

  9. Oerum S, Meynier V, Catala M, Tisné C. A comprehensive review of m6A/m6Am RNA methyltransferase structures (2021). Methods Mol Biol. 2021;2298:307-323. doi: 10.1007/978-1-0716-1374-0_19.

  10. Mboukou A, Rajendra V, Kleinova R, Tisné C, Jantsch MF, Barraud P. Transportin-1: A Nuclear Import Receptor with Moonlighting Functions (2021). Front Mol Biosci. 18;8:638149. doi: 10.3389/fmolb.2021.638149.

  11. Yoluç Y, Ammann G, Barraud P, Jora M, Limbach PA, Motorin Y, Marchand V, Tisné C, Borland K, Kellner S. Instrumental analysis of RNA modifications (2021). Crit Rev Biochem Mol Biol. 2021 Apr;56(2):178-204. doi: 10.1080/10409238.2021.1887807.

  12. S. Oerum, M. Catala, M. Bourguet, L. Gilet, P. Barraud, S. Cianférani, C. Condon, C. Tisné. Structural studies of RNase M5 reveal two-metal-ion supported two-step dsRNA cleavage for 5S rRNA maturation (2021). doi: 10.1080/15476286.2021.1885896

  13. S. Oerum, T. Dendooven, M. Catala, L. Gilet, C. Dégut, A. Trinquier, M. Bourguet, P. Barraud, S. Cianferani, B. F. Luisi, C. Condon*, C. Tisné*, Structures of B. subtilis maturation RNases captured on 50S ribosome with pre-rRNAs, Mol Cell (2020), doi: 10.1016/j.molcel.2020.09.008

  14. P. Barraud*, A. Gato, M. Heiss, M. Catala, S. Kellner & C. Tisné*, Time-resolved NMR monitoring of RNA maturation, Nature Communications 10 (2019), p. 3373-3387. doi: 10.1038/s41467-019-11356-w

  15. A. Mouhand, M. Pasi, M. Catala, L. Zargarian, A. Belfetmi, P. Barraud, O. Mauffret, C. Tisné. Viruses. Overview of the nucleic-acid binding properties of the HIV-1 nucleocapsid protein in its different maturation states, Viruses (2020), doi: 10.3390/v12101109

  16. M. Catala, A. Gato, C. Tisné & P . Barraud*, Yeast tRNAPhe samples preparation for NMR spectroscopy, (2020) Bio-protocol doi: 10.21769/BioProtoc.3646

  17. P. Sun, C. Dégut, S. Réty, J-C Caissard, L. Hibrand-Saint Oyant, A. Bony, S. N. Paramita, C. Conart, J-L Magnard, J. Jeauffre, A. M. Abd-El-Haliem, J. Marie-Magdelaine, T. Thouroude, R. Baltenweck, C. Tisné, F. Foucher, M. Haring, P. Hugueney, R. C. Schuurink & S. Baudino, Functional diversification in the Nudix hydrolase gene family drives sesquiterpene biosynthesis in Rosa x wichurana, Plant Journal (2020), doi: 10.1111/tpj.14916

  18. M. Catala, A. Gato, C. Tisné & P . Barraud*, 1H, 15N chemical shift assignments of the imino groups of yeast tRNAPhe: influence of the post-transcriptional modifications, Biomol NMR assign (2020), doi:10.1007/s12104-020-09939-6.

  19. C. Dégut, V. Schwarz, L. Ponchon, P. Barraud, R. Micura & C. Tisné, Design of covalent RNA/protein complexes for structural studies, Biochimie (2019), doi: 10.1016/j.biochi.2019.03.021

  20. C. Dégut#, M. Roovers#, P. Barraud, F. Brachet, A. Feller, V. Larue, A. Al Refaii, J. Caillet, L. Droogmans*, C. Tisné*, Structural characterisation of B. subtilis m1A22 tRNA methyltransferase TrmK: Insights into tRNA recognition, Nucleic Acids Research (2019), doi: 10.1093/nar/gkz230

  21. P. Barraud* & C. Tisné, To be or not to be modified: miscellaneous aspects influencing nucleotide modifications in tRNAs, IUBMB life (2019), doi: 10.1002/iub.2041

  22. S. Oerum#, M. Catala#, C. Atdjian, F. Brachet, L. Ponchon, P. Barraud, L. Iannazzo, L. Droogmans, E. Braud, M. Ethève-Quelquejeu & C. Tisné*, Bisubstrate analogues as structural tools to investigate m6A methyltransferase active sites, RNA Biology (2019), doi: 10.1080/15476286.2019.1589360

  23. P. Asadi-Atoi, P. Barraud, C. Tisné and S. Kellner*, Benefits of stable isotope labeling in RNA analysis. Biological Chemistry (2019), doi: 10.1515/hsz-2018-0447

  24. C. Bou-Nader, P. Barraud, L. Pecqueur, S. Sacquin-Mora, J. Pérez, C. Velours, M. Fontecave, C. Tisné & D. Hamdane*, Molecular basis for transfer RNA substrate recognition by human dihydrouridine synthase. Nucleic Acids Res. (2019), doi: 10.1093/nar/gky1302

  25. D. Schweida, P. Barraud, C. Regl, F.E. Loughlin, C. G. Hubert, C. Cabrele, M. Schubert, The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins, J. Biomol NMR, 73(2019), p. 71-79.

  26. S. Lyonnais, S. K. Sadiq, C. Lorca-Oró, L. Dufau, S. Nieto-Marquez, T. Escriba, N. Gabrielli, X. Tan, M. Ouizougun-Oubari, J. Okoronkwo, M. Reboud-Ravaux, J. Maria Gatell, R. Marquet, J.-C. Paillart, A. Meyerhans, C. Tisné, R. J. Gorelick, G. Mirambeau, The HIV-1 ribonucleoprotein dynamically regulates its condensate behavior and drives acceleration of protease activity through membrane-less granular phase-separation, bioRxiv (2019), doi: 10.1101/528638

  27. A. Mouhand§, A. Belfetmi§, M. Catala, V. Larue, L. Zargarian, F. Brachet, R. J. Gorelick, C. Van Heijenoort, G. Mirambeau, P. Barraud, O. Mauffret & C. Tisné*, Modulation of the HIV nucleocapsid dynamics finely tunes its RNA-binding properties during virion genesis. Nucleic Acids Res, 46 (2018), p. 9699-9710.

  28. S. E. El Meshri, E. Boutant, A. Mouhand, A. Thomas, V. Larue, L. Richert, V. Vivet-Boudout, Y. Mély, C. Tisné*, D. Muriaux* & H. de Rocquigny*, The NC domain of HIV-1 Gag contributes to the interaction of Gag with TSG101. BBA general subject 1862 (2018), p. 1421-1431

  29. V. Larue*, M. Catala, A. Belfetmi, L. Zargarian, O. Mauffret & C. Tisné*, 1H, 13C and 15N backbone and partial side-chain resonance assignments of the C-terminal domain of HIV-1 Pr55Gag encompassed in NCp15. Biomol NMR Assign 12 (2018), p. 139-143.

  30. Beusch, P. Barraud*, A. Moursy, A. Cléry, F.H.-T. Allain*. Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7. eLife (2017) e25736.

  31. Oerum S., Dégut C., Barraud P., Tisné C. m1A Post-Transcriptional Modification in tRNAs Biomolecules 7 (2017) E20.

  32. H. Guyon, F. Mavré, M. Catala, S. Turcaud, F. Brachet, B. Limoges*, C. Tisné* & L. Micouin*, Use of Redox Probe for Electrochemical RNA-ligand Binding Assay in Microliter Droplets, Chem Comm 53 (2017), 1140-1143.

  33. C. Dégut, P. Barraud*, V. Larue, C. Tisné*, Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis. Biomolecular NMR assignment (2016) 10 (2016), p. 253-257.

  34. A. Belfetmi, L. Zargarian, C. Tisné, D. Sleiman, N. Morellet, E. Lescop, O. Maskri, B. René, Y. Mély, P. Fossé, O. Mauffret, Insights into the mechanisms of destabilization of RNA secondary structure by the HIV-1 nucleocapsid protein. RNA 22 (2016), p. 506-517.

  35. C. Dégut, L. Ponchon, M. Folly-Klan, P. Barraud & C. Tisné*, The m1A58 modification in eubacterial tRNA: an overview of tRNA recognition and mechanism of catalysis by TrmI. Biophys. Chemistry 210 (2016), 27-34.

  36. C. Dégut, A. Monod, F. Brachet, C. Crépin & C. Tisné*, In vitro/in vivo production of tRNA for X-ray studies. Methods Mol Biol 1320 (2016), p. 37-57.

  37. L. Iannazzo, E. Benedetti, M. Catala, M. Ethève-Quelquejeu, C. Tisné, L. Micouin. Monitoring of reversible broronic acid-diol interactions by fluorine NMR spectroscopy in aqueous media. Org. Biomol. Chem. 13 (2015), p. 8817-8821.

  38. M. E. Khouri, M. Catala, B. Seijo, J. Chabal, C. Tisné, F. Dardel & Ponchon L., Expression and purification of RNA-Protein complexes in Escherichia coli. Methods Mol Biol. 1316 (2015), p. 25-31.

  39. A. Blond, E. Ennifar*, C. Tisné* & L. Micouin*, The design of RNA binders : targeting the HIV replication cycle as a case study. ChemMedChem 9 (2014), p. 1982-1996.

  40. M. Mori, A. Nucci, M. Chiara Dasso Lang, N. Humbert, C. Boudier, F. Debaene, S. Sanglier-Cianferani, M. Catala, P. Schult-Dietrich, U. Dietrich, C. Tisné, Y. Mély & M. Botta, Functional and Structural Characterization of a 2-amino-4-phenylthiazole inhibitor of the HIV-1 Nucleocapsid Protein with Antiviral Activity. ACS Chem Biol 9 (2014), p. 1950-1955

  41. L. Zargarian, C. Tisné, P. Barraud, N. Morellet, B. René, Y. Mély, P. Fossé & O. Mauffret, The distinct roles of the two zinc fingers of the HIV-1 nucleocapsid protein could be modulated by its linker residues. PloS ONE 9 (2014), e102150.

  42. D. Sleiman, S. Bernacchi, S. X. Guerrero, F. Brachet, V. Larue, J.-C. Paillart* & C. Tisné*, Characterization of RNA binding and chaperoning activities of HIV-1 Vif protein: importance of the C-terminal unstructured tail. RNA Biol 11 (2014), p. 906-920.

  43. L. Ponchon, M. Catala, B. Seijo, M. El Khouri, F. Dardel, S. Nonin-Lecomte & C. Tisné, Co-expression of RNA/protein complexes in Escherichia coli and applications to RNA biology. Nucleic Acids Res, 41 (2013), e150.

  44. D. Sleiman, P. Barraud, F. Brachet & C. Tisné*, The interaction between tRNALys3 and the primer activation signal deciphered by NMR spectroscopy. PLoS ONE, 8 (2013): e64700.

  45. J. Piton, V. Larue, Y. Thillier, A. Dorléans, O. Pellegrini, I. Li de la Sierra-Gallay, J.J. Vasseur, F. Debart, C. Tisné & C. Condon, The B. subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates. PNAS, 110 (2013), p. 8858–8863.

  46. T. Lombès, R. Moumné, V. Larue, E. Prost, M. Catala, T. Lecourt, F. Dardel, L. Micouin* & C. Tisné*, Investigation of RNA-ligand interaction by 19F NMR spectroscopy using fluorinated probes. Angewandte Chemie Int. Ed. Engl. 51 (2012), p. 9530-9534 .

  47. D. Sleiman, V. Goldschmidt, P. Barraud, R. Marquet, J.-C. Paillart & C. Tisné*, Initiation of HIV-1 reverse transcription and functional role of nucleocapsid-mediated tRNA/viral genome interactions. Virus Research 169 (2012), p. 324-339.

  48. J. Batisse, S. Guerrero, S. Bernacchi, D. Sleiman, C. Gabus, J.-L. Darlix, R. Marquet, C. Tisné, J.-C. Paillart, The role of Vif oligomerization and RNA chaperone activity in HIV-1 replication. Virus Research 169 (2012), p. 361-376.

  49. R. Moumné, M. Catala, V. Larue, L. Micouin & C. Tisné*, Fragment-based design of small RNA binders: promising developments and contribution of NMR. Biochimie 94 (2012), p. 1607-1619.

  50. A. Guelorget, P. Barraud, C. Tisné & B. Golinelli-Pimpaneau, Structural comparison of tRNA m1A58 methyltransferases revealed different molecular strategies to maintain their oligomeric architecture under extreme conditions. BMC Structural Biology 11 (2011), p. 48.

  51. R. Moumné, M. Pasco, E. Prost, T. Lecourt, L. Micouin* & C. Tisné*, Fluorinated diaminocyclopentanes as chiral sensitive probes of RNA structure. J. Am. Chem. Soc. 132 (2010), p. 13111-13113.

  52. R. Moumné, V. Larue, B. Seijo, T. Lecourt, L. Micouin* & C. Tisné*, Tether influence on the binding properties of tRNALys3 ligands designed by a fragment based approach. Org. Biomol. Chem. 8 (2010), p. 1154-1159.

  53. F Chung, C. Tisné*, T. Lecourt, B. Seijo, F. Dardel & L. Micouin*, Design of tRNALys3 ligands: fragment evolution and linker selection guided by NMR spectroscopy. Chemistry, a European journal 15 (2009), p. 7109-7116.

  54. V. Larue, B. Seijo, C. Tisné & F. Dardel, 1H, 13C and 15N NMR assignments of the E. coli peptide deformylase in complex with a natural inhibitor called actinonin. Biomolecular NMR assignments 3 (2009), p. 153-155.

  55. P. Barraud, E. Schmitt, Y. Mechulam, F. Dardel & C. Tisné*, A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis. Nucl. Acids Res., 36 (2008), p. 4894-4901.

  56. P. Barraud, J.C. Paillart, R. Marquet & C. Tisné*, Advances in the structural understanding of Vif proteins. Curr. HIV Res., 6 (2) (2008), p. 91-99.

  57. P. Barraud, B. Golinelli-Pimpaneau, C. Atmanene, S. Sanglier, A. Van Dorsselauer, L. Droogmans, F. Dardel & C. Tisné*, Crystal structure of Thermus thermophilus tRNA m1A58 methyltransferase and biophysical characterization of its interaction with tRNA. J. Mol. Biol., 377 (2008), p. 535-550.

  58. P. Barraud, C. Gaudin, F. Dardel & C. Tisné*, New insights into the formation of HIV-1 reverse transcription initiation complex. Biochimie, 89 (2007), p. 1204-1210.

  59. P. Barraud, F. Dardel & C. Tisné*, Optimizing HSQC experiment for the observation of exchange broadened signals in RNA-protein complexes. C R Chimie, 11 (2008), p. 91-99.

  60. F Chung, C. Tisné*, T. Lecourt, F. Dardel* & L. Micouin*, NMR-guided fragment-based approach for the design of tRNALys3 ligands. Angewandte Chemie Int. Ed. Engl., 46(2007), p. 4489-4491.

  61. C. Tisné*, F. Guillière & F. Dardel, NMR-based identification of peptides that specifically recognize the D-arm of tRNA. Biochimie, 87(2005), p. 885-888.

  62. C. Tisné*, Structural bases of the annealing of HIV-1 reverse transcription primer tRNALys3 to the viral RNA. Curr. HIV Res., 3 (2)(2005), p. 147-156.

  63. C. Tisné, B. P. Roques & F. Dardel, The annealing mechanism of HIV-1 reverse transcription primer onto the viral genome. J. Biol. Chem., 279 (2004), p. 3588-3595.

  64. C. Gaudin, S. Nonin-lecomte, C. Tisné, S. Corvaisier, V. Bordeau, F. Dardel & B. Felden, The tRNA-like domains of E. coliand A. aeolicus transfer messenger RNA : Structural and functional studies. J. Mol. Biol. 331(2003), p. 457-471.

  65. C. Tisné*, B. P. Roques & F. Dardel, Specific recognition of primer tRNALys3 by HIV-1 nucleocapsid protein: involvement of the zinc fingers and of the N-terminal basic extension. Biochimie, 85 (2003), p. 557-561.

  66. C. Tisné & F. Dardel, Optimisation of a peptide library for screening specific RNA ligands by flow-injection NMR. Comb. Chem. High Troughput Screen., 5(2002), p513-518.

  67. C. Tisné*, B. P. Roques & F. Dardel*, Heteronuclear NMR studies of the interaction of tRNALys3 with HIV-1 nucleocapsid protein. J. Mol. Biol., 306 (2001), p. 443-454.

  68. B. Brutscher, J. Boisbouvier, E. Kupce, C. Tisné, F. Dardel, D. Marion & J.-P. Simorre, Base-type-selective high resolution 13C edited NOESY for sequential assignment of large RNA. J. Biomol. NMR., 19 (2001), p. 141-151.

  69. C. Tisné, M. Rigourd, R. Marquet, C. Ehresmann & F. Dardel, NMR and biochemical characterisation of recombinant human tRNALys3 expressed in E. coli: Identification of post-transcriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription. RNA, 6 (2000), p. 1403-1412.